Total antioxidant status (TAS) and Total oxidant status (TOS) kits: Biotechno Labs
Oxidative stress is the imbalance between the free radicals and the antioxidants in the body. It can be also said that it is an imbalance between the accumulation and production of Reactive Oxyegen species (ROS). For understanding the oxidative stress in any diseased condition like cancer, the assessment of oxidants and anti-oxidants is essential. Hence to support the scientist in their research Biotechno Labs are providing kits for assessing these two: Total antioxidant status (TAS) and Total oxidant status(TOS) kits. Both of them are colormetric kits.
In Total antioxidant assay kit, ABTS is oxidized to green ABTS•+ by appropriate oxidant, which can be reduced to colourless ABTS in the presence of antioxidants. The TAS of the sample can be determined and calculated by measuring the absorbance of ABTS•+ at 660 nm. Trolox is an analog of VE and has a similar antioxidant state to that of VE. Trolox is used as a reference substance for total antioxidant status.
In Total oxidant assay kit, under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2–3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample.
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